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Lipidomics, the study of lipid profiles in biological systems, has emerged as a promising field for the identification of functional biomarkers. Human saliva, a readily accessible biofluid, offers several advantages for biomarker research. This article provides an overview of the analytical strategies employed in lipidomics for the identification of functional biomarkers from human saliva. The article discusses sample collection and preparation techniques, lipid extraction methods, analytical techniques such as mass spectrometry and chromatography, and data analysis approaches. It highlights the potential of lipidomic analysis in saliva for understanding disease mechanisms and advancing personalized medicine.